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95
Sino Biological mouse monoclonal antibody against influenza b victoria lineage ha
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
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Cusabio antibodies against ha tag
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
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Santa Cruz Biotechnology abs against mouse total
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
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Transgene Biotek mouse or rabbit antibodies against gst or his, histone h3, ha, flag, or strep
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
Mouse Or Rabbit Antibodies Against Gst Or His, Histone H3, Ha, Flag, Or Strep, supplied by Transgene Biotek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse monoclonal antibody directed against the ha-tag
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
Mouse Monoclonal Antibody Directed Against The Ha Tag, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse monoclonal antibody against ha-tag 2-2.2.14
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
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Thermo Fisher mouse monoclonal antibody against ha
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
Mouse Monoclonal Antibody Against Ha, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc antibody against ha tag
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
Antibody Against Ha Tag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc antibodies against ha
Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and <t>B/Victoria.</t> Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.
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Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and B/Victoria. Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.

Journal: mBio

Article Title: A decavalent composite mRNA vaccine against both influenza and COVID-19

doi: 10.1128/mbio.00668-24

Figure Lengend Snippet: Design and characterization of 10-valent mRNA vaccine (FLUCOV-10). ( A ). Schematic illustration of the FLUCOV-10 formulation, a 10-valent combination mRNA vaccine targeting both influenza and COVID-19. It includes mRNAs encoding the full-length HA proteins from influenza A virus subtypes A/H1, A/H3, A/H5, and A/H7, and from influenza B virus lineages B/Yamagata and B/Victoria. Additionally, it encodes full-length spike proteins from SARS-CoV-2 variants Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5. Each mRNA component is individually encapsulated in lipid nanoparticles (LNPs) prior to being combined into the final FLUCOV-10 formulation. (B and C) Phylogenic trees were created for influenza HAs ( B ) and SARS-CoV-2 spikes ( C ) by using Nextstrain. The vaccine HAs or spike are indicated with red triangles, and the challenge viruses are indicated with an “X.” (D) The expression of FLUCOV-10-mRNA-encoded HA proteins in 293T cells was determined by western blotting. Lane 1, 293T cells with mock transfection; lane 2, 293T cells with indicated mRNA transfection. (E) The expression of FLUCOV-10-mRNA-encoded spike proteins. Lane 1, 293T cells with mock transfection; lane 2–5, 293T cells with Wuhan-Hu-1, BQ.1.1, BA.2.75.2, and XBB.1.5 mRNA transfection, respectively. β-Actin was used as western blotting loading control. (F) Particle size distribution of individual LNP formulations was measured in triplicate using dynamic light scattering (DLS) on the NS-90Z Nanoparticle Size and Zeta Potential Analyzer.

Article Snippet: The HA and spike proteins in cell lysates were then detected by western blotting using a mouse monoclonal antibody against SARS-CoV-2 spike proteins (GTX632604, GeneTex), a rabbit polyclonal antibody against influenza A/H1 HA (11055-T62, Sino Biological), a mouse monoclonal antibody against influenza A/H3 HA (11056-MM03, Sino Biological), a rabbit polyclonal antibody against influenza A/H5 HA (11062-T62, Sino Biological), a rabbit polyclonal antibody against influenza A/H7 HA (40103-T62, Sino Biological), a rabbit polyclonal antibody against influenza B/Yamgata lineage HA (11053-T62, Sino Biological), and a mouse monoclonal antibody against Influenza B/Victoria lineage HA (11053-MM06, Sino Biological). β-Actin was detected using anti-β-actin antibody.

Techniques: Formulation, Virus, Expressing, Western Blot, Transfection, Control, Zeta Potential Analyzer